See every molecule. Reveal what ensemble methods hide. Your benchtop just got Exciting.
Ensemble methods blur the detail. They average out the rare conformations, transient states and molecular subpopulations that drive your biology. You deserve to see what's really happening.
The EI-FLEX Pro is a confocal benchtop system that reveals single-molecule behaviour in free solution — no surface immobilisation, no darkroom, no PhD in optics required. Just pipette, measure and discover.
Resolve hidden heterogeneity and rare conformations that ensemble averages miss. See what's really going on — one molecule at a time.
No darkroom. No optical table. No specialist training. Plug in, pipette, and start collecting publication-ready data before lunch.
Our team of single-molecule specialists will help you design experiments, run proof-of-concept samples, and get the most from your EI-FLEX Pro.
Start with coverslips. Upgrade to 96 or 384-well plates when throughput matters. One platform that grows with your research.
Every application below runs on a single EI-FLEX Pro. No swapping instruments, no re-training. Just different questions, same platform.
PROTACs and molecular glues need stable ternary complexes to work, but ensemble methods can't tell you if one actually formed — or how long it lasts.
The EI-FLEX Pro detects ternary complex formation at single-molecule resolution in free solution, giving you binding stoichiometry, stability and kinetics in a single experiment.
smFRET · FCCSYour protein exists in multiple states. Ensemble averages hide them. You need to see each conformation and how they interconvert.
Freely diffusing smFRET reveals distinct conformational populations, their relative proportions, and transition rates — without surface artefacts.
smFRET-ALEXYou need to know if your antibody binds, how tightly, and whether it changes the target's conformation — all with minimal sample.
FCS and FCCS measure binding affinity and co-diffusion at picomolar concentrations. Add smFRET to see conformational consequences of binding.
FCS · FCCS · smFRETDNA/RNA-binding proteins are central to gene regulation, but you can't study their dynamics with gels and blots.
Measure binding, unbinding and conformational rearrangements of protein–nucleic acid complexes in real time with smFRET and burst analysis.
smFRET · BURST SEARCHAggregation is a dealbreaker for biologics — but early-stage oligomers are invisible to DLS and SEC.
FCS detects changes in diffusion time as monomers assemble into oligomers, catching aggregation events that bulk methods miss entirely.
FCS · FCCSYour drug candidate binds, but does it lock the target in the right state? You need structural context, not just a binding curve.
smFRET with ALEX excitation maps distance changes across labelling sites, revealing how drug binding reshapes the conformational landscape.
smFRET-ALEXMost instruments solve one step. We built a platform that connects all of them — so you spend less time stitching workflows together and more time doing science.
Our tools and team help you identify optimal labelling positions — maximising signal without disrupting your molecule's native behaviour.
LABEL DESIGNFrom dye selection to conjugation strategy, we'll guide you through sample preparation that works first time — or run it for you.
SAMPLE PREPThe EI-FLEX Pro does the heavy lifting. Confocal detection, automated acquisition, and real-time single-molecule sensitivity — all on your benchtop.
EI-FLEX PROIntegrated software takes you from raw photons to publication-ready figures. FRET histograms, FCS curves, burst analysis — no third-party tools required.
INTEGRATED SOFTWAREAcquisition, laser control, FCS analysis and smFRET burst search — all in one place, all intuitive.
Real outputs from the EI-FLEX Pro. No post-processing, no external software. Just clear, Exciting results.
Two distinct conformational populations, resolved beautifully at the single-molecule level using ALEX excitation.
TECHNIQUE: smFRET-ALEXDiffusion times and binding kinetics extracted directly from fluorescence fluctuations. Clean curves, real insight.
TECHNIQUE: FCSEvery dot is a molecule. Individual photon bursts identified, classified and ready for your next figure.
TECHNIQUE: BURST SEARCH
"What sets EI-FLEX apart is its versatility — it's our go-to for probing kinetics and conformational changes that other systems can't fully resolve."
"Solution-based measurements avoid surface tethering artefacts. You label, dilute, and measure — results in minutes rather than days with TIRF systems."
"It has opened up new avenues of research because of the combined FCS, FCCS and single-molecule FRET capabilities. A game-changer for us."
When Dr. Quinn's group switched from TIRF, everything changed. Here's what happened.
"We used to spend three weeks on a single TIRF-based smFRET dataset — surface prep, alignment, troubleshooting. With the EI-FLEX Pro, we go from labelled sample to a complete FRET histogram in under an hour. It's fundamentally changed how we design experiments."
No darkroom. No alignment nightmares. Just great science, faster.
Add your labelled sample to a well plate or coverslip. Picomolar concentrations, microlitre volumes — so even your most precious preps go further.
~2 minutesThe software guides you through setup and runs the measurement. Single molecules, detected one at a time, as they move freely in solution. No alignment, no tweaking.
10–30 minutesYour results are ready the moment acquisition ends — histograms, correlation curves, burst analysis, all built in and figure-ready. From sample to insight in an afternoon.
InstantBook a live demo with our team, or send us your sample — we'll run a proof-of-concept measurement for free.